Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Lizard Embryonic Fibroblast Cell Line (ASEC-1)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Embryo |
| Organism |
Lizard (Dactyloidae) |
| Donor History |
Male ,Stage 6 embryo (torso) ,Anolis sagrei |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. DMEM ,High Glucose Medium (TM500) + 10% HI-FBS + 2mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,29.0°C ,5% CO₂.Note: cells are grown at 29.0°C. Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. |
| Immortalization Method |
Spontaneously immortalized in culture. |
| Full Information |
This immortalized lizard embryonic friboblast cell line is derived from primary fribroblast cells isolated from the torso (with internal organs removed) of a single stage 6 Anolis sagrei embryo. The primary cells were serially passaged until signs of cell growth ended (passage 26). After several days of continued culture, a small number of cell resumed proliferating, and an individual cell from this culture was isolated to generate the ASEC-1 cell line.
