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Imunoanalýza
Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Brain |
| Organism |
Mouse (M. musculus) |
| Donor History |
Neonatal mouse (WT 129 Sv strain) ,Cerebral Cortex |
| Growth Properties |
Adherent ,spindle-shaped |
| Growth Conditions | |
| Immortalization Method |
Transformation with oncoprotein of murine Polyomavirus ,Polyoma middle T antigen (PymT) |
| Full Information |
https://www.abmgood.com/immortalized-mouse-cerebral-capillary-endothelial-cells-cend.html |
Homeostasis of the central nervous system (CNS) is maintained by the blood brain barrier (BBB) and disruptions to the BBB are linked to many disorders of the CNS. The Immortalized Mouse Cerebral Capillary Endothelial Cell Line (cEND) provides a useful model for studies involving the differentiation and regulation of BBB as it shares principal features of the BBB in vivo, specifically in that 1) it retains the endothelial markers VE-Cadherin and PECAM-1; 2) it displays tight junction associated markers such as occludin, claudin-3, -5, -12, 3) it expresses the BBB marker protein Glut-1 and 4) it shows high electrical resistance, representing barrier properties. This cell line is also responsive to glucocorticoid, estrogen-treatment and pro-inflammatory mediator such as TNFα, making this cell line valuable in elucidating cellular responses of endothelial cells to different stimuli. Together with the Immortalized Mouse Cerebellar Capillary Endothelial Cell Line (cerebEND), the two cell lines represent important in vitro model system for these different brain regions.
