Immortalized Mouse Colonic Epithelial Cells – Conditionally (YAMC)

YAMC is a conditionally immortalized mouse colon epithelial cell line isolated from the mouse harboring thermolabile mutation (tsA58) under the control of an interferon (IFN)-γ-inducible H-2Kb promoter and a temperature-sensitive simian virus 40 large T antigen. At a non-permissive temperature (37°C-39°C), the cells cease to proliferate and begin to detach from the plate. Cells in culture display some microvilli formation and expression levels of the brush border-associated enzymes, alkaline phosphatase, dipeptidyl peptidase IV, and sucrase, can be induced in the line by the addition of 1mM sodium butyrate, 1 µM Phorbol Myristate Acetate (PMA), or 1 µM Retanoic Acid (RA) to the culture medium for 5 days. Conversely, the addition of 5 µg/ml TGF-β or 1 µM Bt2GMP will downregulate the two enzymes. Immunohistochemical staining was used to detect the presence of SV40 large T antigen and keratin 18.