Immortalized Mouse Dental Apical Papilla Progenitor Cells (iSCAP)

The cells are unique, as the immortalization process may be reversed by infection with Cre-recombinase expressing adenoviruses (abm Cat. No. 000018A), thus reverting immortal cells back into primary cells. When transduced with adenovirus expressing BMP9, these cells are able to differentiate into osteogenic, chondrogenic, and adipogenic lineages. Besides expressing osteoblastic/odontoblastic Expression such as OPN, OCN, BSP, MEPE, DMP1, DSPP, and ALP, these cells are also able to form mature and mineralized osteoid matrix upon BMP9-transductionThese cells are recommended for use in studies involving apical papilla stem cell biology, and ondontogenic tissue engineering.