Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Porcine Fibroblast Cell Line (iKUF03)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Ear |
| Organism |
Pig (Porcine) |
| Donor History |
Ear ,NIH miniature pigs |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Transfection with human telomerase reverse transcriptase (hTERT) and subsequent serial passaging. |
| Full Information |
https://www.abmgood.com/Immortalized-Porcine-Fibroblast-Cell-Line-iKUF03-t0392.html |
The iKUF03 cell line originates from ear tissue of an NIH miniature pig, making it valuable for research due to the NIH pig’s well-documented genetic background. Immortalized using hTERT, this cell line is characterized by a specific and homogenous SLA genotype, crucial for detailed immunogenetic studies. It provides a stable platform for examining MHC class I and II interactions and has been maintained through 35 passages, showing consistent growth and typical fibroblast morphology. Additional cell lines from this panel:T0390 – Immortalized Porcine Fibroblast Cell Line (iKUF01)T0391 – Immortalized Porcine Fibroblast Cell Line (iKUF02)T0392 – Immortalized Porcine Fibroblast Cell Line (iKUF03)T0393 – Immortalized Porcine Fibroblast Cell Line (iKUF04)T0394 – Immortalized Porcine Fibroblast Cell Line (iKUF07)T0395 – Immortalized Porcine Fibroblast Cell Line (iKUF09)
