Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Porcine Fibroblast Cell Line (iKUF04)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Muscle |
| Organism |
Pig (Porcine) |
| Donor History |
NIH miniature pig |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. Drug resistance: Puromycine (2ug/ml) |
| Immortalization Method |
Transfection with human telomerase reverse transcriptase (hTERT) and subsequent serial passaging. |
| Full Information |
https://www.abmgood.com/Immortalized-Porcine-Fibroblast-Cell-Line-iKUF04-t0393.html |
The iKUF04 cell line is derived from ear tissue of an NIH miniature pig and has a unique distinction of being homozygous across all tested SLA loci (SLA-1, SLA-2, SLA-3, DQA, DQB1, and DRB1), which is rare and immensely valuable for controlled immunological studies. This homozygosity makes it an ideal candidate for studying the effects of specific SLA alleles on immune responses. The cell line has been immortalized with hTERT and cultured up to 30 passages, maintaining stable growth and characteristic fibroblast features.Additional cell lines from this panel:T0390 – Immortalized Porcine Fibroblast Cell Line (iKUF01)T0391 – Immortalized Porcine Fibroblast Cell Line (iKUF02)T0392 – Immortalized Porcine Fibroblast Cell Line (iKUF03)T0393 – Immortalized Porcine Fibroblast Cell Line (iKUF04)T0394 – Immortalized Porcine Fibroblast Cell Line (iKUF07)T0395 – Immortalized Porcine Fibroblast Cell Line (iKUF09)
