Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Rat Bone Cell Line (IRC10-30 Myc-3)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Bone |
| Organism |
Rat (R. norvegicus) |
| Donor History | |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow VIII Medium (TM018) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂ |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/Immortalized-Rat-Bone-Cell-Line-IRC10-30-Myc-3-t0217.html |
These novel bone cell lines were established by immortalizing an osteoblastic clonal cell population with avian v-mycOK10 gene. Although both cells are from the same clonal population, T0216 and T0217 have differing properties. T0217 does not express AP, shows no PTH responsiveness, and synthesizes only about one-third as much collagen as T0216 (4 versus 12% of total protein synthesis). However, T0217 can be induced to synthesize cAMP by PGE2 and produces osteocalcin mRNA. Cells are osteogenic, form cartilage and fibrous tissue, and have multipotential differentiation capacity and form bone and cartilage in vivo.
