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Imunoanalýza
Immortalized Rat Luteal Cells – Conditionally (GG-CL)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Ovary |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Day 14 pregnant Sprague-Dawley rat |
| Growth Properties |
Adherent ,flat |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,33.0°C ,5% CO₂ |
| Immortalization Method |
Serial passaging and infection by SV-40 tsA209 Temperature Sensitive Mutant virus |
| Full Information |
https://www.abmgood.com/immortalized-rat-luteal-cells-conditionally-gg-cl.html |
The corpus luteum develops from an ovarian follicle during the luteal phase of the menstrual cycle and is responsible for the decidualization of the endometrium. The Immortalized Rat Luteal Cells (GG-CL) is conditionally immortalized through the temperature-sensitive simian virus (SV40 tsA209) and has retained many primary luteal cell markers such as estrogen receptor-β, glucocorticoid receptor (GR), interleukin-6 (IL-6), orphan nuclear receptor nur 77 and 20α-hydroxysteroid dehydrogenase (20α-HSD). In addition, GG-CL responds to stimulation by PRL, estradiol, and cAMP, making the cells a useful model for studies involving gene expression and regulation of luteal cells. Northern analysis and RT-PCR were used to confirm the expression of IL-6, glucocorticoid receptor (GR), estrogen receptor-β (ER-β) and orphan nuclear receptor nur 77.
