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Immortalized Rat Neural Stem Cells (1464R)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Brain |
| Organism |
Rat (R. norvegicus) |
| Donor History |
12 weeks old |
| Growth Properties |
Suspension |
| Growth Conditions |
Cultures can be grown on poly(2-hydroxyethylmethacrylate) (PHEMA ,#P3932; Sigma) coated cell culture vessels to prevent cell attachment. Neurobasal medium (Gibco) + 2 mM L-Glutamine (G275) + 2% B27 supplement (Thermo Fisher Scientific ,Waltham ,CA ,USA) + 10 ng/mL of FGF2 (Z101455) + 10 ng/mL of EGF (Z100139) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. For information regarding cell differentiation ,please refer to the "Differentiation Protocol" PDF under the Documents Tab. |
| Immortalization Method |
Spontaneous immortalization |
| Full Information |
https://www.abmgood.com/immortalized-rat-neural-stem-cells-1464r.html |
Immortalized Rat Neural Stem Cells (1464R) are spontaneously immortal population of neural stem cells derived from the brain stem. The cells retained morphology even after 50 passages. In the presence of retinoic acid, the cells may differentiate to TuJ1-positive neurons and to GFAP-positive astrocytes or O4-positive oligodendrocytes. May be used for brain disease studies, and a source for neuronal and glial cells.
