Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Rat Retinal Cells (E1A-NR.3)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Retina |
| Organism |
Rat (R. norvegicus) |
| Donor History |
PN6 Sprague-Dawley Rat |
| Growth Properties |
Adherent ,neuronal |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂ |
| Immortalization Method |
Serial passaging and transduction with retroviruses carrying 12S portion of replication incompetent adenovirus E1A gene |
| Full Information |
https://www.abmgood.com/immortalized-rat-retinal-cells-e1a-nr-3.html |
Immortalized Rat Retinal Cells (E1A-NR.3) are immortalized with the 12S portion of replication incompetent adenovirus E1A gene. It expresses vimentin and is the parental cells to the Immortalized Rat Retinal Precursor Cell Line (R28). E1A-NR.3 cells exhibit contact inhibition and anchorage-dependent growth. Researchers may use this cell line to study photoreceptor rescue or replacement paradigm as studies have shown that the E1A-NR.3 cells are capable to successfully be transplanted intraocularly. Immunoreactivity was assessed in vitro and in vivo for markers.
