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Imunoanalýza
IPC-366 Cells
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Mammary |
| Organism |
Dog (Canine) |
| Donor History |
Female ,Inflammatory Mammary Cancer |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 10% FBS + 2 mM L-glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂ |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
IPC-366 Cells are derived from a triple negative ( ER-, PR-, HER-2-) canine primary inflammatory mammary cancer (IMC) sample that was obtained after the euthanasia of the clinically diagnosed female dog. The morphology of these cells when adhered is epithelial-like and they are positive for general epithelial markers (pancytokeratin, AE1/AE3) as well as the basal marker CK14. Cells also over express E-cadherin and COX-2 and have a high rate of proliferation, but lack p63 and smooth muscle actin. IPC-366 Cells share several characteristics to human derived IMC line, SUM149, including its triple negative nature, and epithelial-like morphology. Due to these similarities, IPC-366 Cells may be useful as a model for study of inflammatory breast cancer.
