JEG-3 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Reproductive system cancer cell lines

Organism

Human

Donor History

Choriocarcinoma

Growth Properties

Adherent

Growth Conditions

Culture medium: EMEM (MEM Eagle)

,

w: 2 mM L-Glutamine

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w: 2.2 g/L NaHCO3

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w: EBSS (Cytion article number 820100a). Supplements: Supplement the medium with 10% FBS and 1% NEAA. Dissociation reagent: Accutase. Seeding density: 2 x 10⁴ cells/cm² will result in a confluent monolayer within 2 to 3 days. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

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or CM-1 (Cytion catalog number 800100)

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which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

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5% CO2

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humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/JEG-3-Cells/300222

SKU: 300222 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The JEG-3 cell line is derived from a human choriocarcinoma, a type of cancer that originates from trophoblastic cells in the placenta. These cells exhibit properties characteristic of trophoblasts, including the ability to produce hormones such as human chorionic gonadotropin (hCG), which is crucial for pregnancy maintenance. JEG-3 cells are epithelial in nature and are often utilized in research focused on placental function, cancer biology, and endocrine signaling.

JEG-3 cells are known for their aggressive growth characteristics and capacity to invade surrounding tissues, making them a valuable model for studying the mechanisms of trophoblastic tumor invasion and metastasis. Additionally, they have been used extensively in research investigating the molecular pathways involved in placental development, as well as the role of trophoblasts in immune tolerance during pregnancy. The cells are typically cultured in RPMI-1640 medium supplemented with fetal bovine serum and other growth factors to support their proliferation and maintenance.

This cell line provides a robust platform for investigating placental cancer biology, hormone production, and the interaction between trophoblasts and the maternal immune system.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.