K-562-GFP Cells

K-562-GFP cells are a genetically modified variant of the human chronic myelogenous leukemia (CML) cell line K-562, originally derived from the peripheral blood of an adult patient in blast crisis. The parental K-562 line is characterized by the presence of the Philadelphia chromosome, resulting in the BCR-ABL fusion protein with constitutive tyrosine kinase activity, which drives uncontrolled proliferation and survival. K-562 cells exhibit erythroleukemia features and can be induced to undergo differentiation along erythroid, megakaryocytic, or monocytic lineages under specific experimental conditions, making them a versatile model for studying hematopoietic differentiation and leukemia biology.

The introduction of green fluorescent protein (GFP) into K-562 cells enables real-time visualization and tracking of leukemic cell behavior in vitro and in vivo. K-562-GFP cells are widely used in assays involving cell proliferation, migration, and drug response, as well as in co-culture systems to study interactions with stromal or immune cells. The fluorescent labeling facilitates applications such as flow cytometry, live-cell imaging, and high-throughput screening.

Genetic modification: Stably modified by replication-incompetent lentiviral transduction to express the ZsGreen1 green fluorescent protein reporter; maintained as a polyclonal population under puromycin selection (1–5 µg/mL). S1/BSL-1 containment.