L6565 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Immortalized Cells

Tissue Type

Mouse cell lines

Organism

Mouse

Donor History
Growth Properties

Adherent and Suspension

Growth Conditions

Culture medium: DMEM:Ham's F12 (1:1)

,

w: 3.1 g/L Glucose

,

w: 2.5 mM L-Glutamine

,

w: 15 mM HEPES

,

w: 0.5 mM Sodium pyruvate

,

w: 1.2 g/L NaHCO3 (Cytion article number 820400a). Supplements: Supplement the medium with 10% FBS

,

0.005 mg/mL insulin

,

0.01 mg/mL human transferrin

,

0.1 mM ethanolamine

,

0.1 mM phosphoethanolamine

,

25 nM selenium

,

500 nM hydrocortisone

,

0.005 mM forskolin

,

bovine pituitary extract (0.15 mg protein per ml). Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/L6565-Cells/305189

SKU: 305189 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

L6565 cells were derived from pancreatic suspensions of splenocytes from L6565 leukemia mice. The chromosomal count ranged from 38 to 144. Electron microscopic observations revealed that the clonal L6565 cells had well-defined nuclei and an abundance of organelles and Class A and Class C viral particles in the cytoplasm. The oncogenes c-myc and c-fos were overexpressed in these cells. The L6565 cell clone is an RNA virus-containing lymphoblastic leukemia stem cell line. It has passed the mycoplasma detection test in this library.

The significance of the L6565 cell line lies in its provision of standardized experimental cell resources and associated technical support for research in the fields of life sciences and biotechnology. These cells can be crucial for understanding the molecular mechanisms of leukemia, particularly the role of viral particles and oncogene expression in leukemogenesis. Additionally, they serve as a valuable tool for drug testing and development, allowing researchers to explore potential therapeutic strategies for leukemia and other related disorders.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.