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LNZ308 Stably Expressing Tet-off Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Brain |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,60 ,Astrocytoma |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% Tet-tested FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 800 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/lnz308-stably-expressing-tet-off-cell-line.html |
LNZ308 cells were transfected with the tTA expression vector to generate the LNZ308 Stably Expressing Tet-off Cell Line. It can be used in vitro for transfected with other tetracycline-responsive expression vectors to create doxycycline-inducible gene expression cells for research. In the presence of doxycycline will turn off gene expression under the tet-regulated promoter.
