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Imunoanalýza
Luciferase Stable U87MG/ΔEGFR Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Brain |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,Glioblastoma |
| Growth Properties |
Adherent ,elongated |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.7 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/luciferase-stable-u87mg-delta-egfr-cell-line.html |
Luciferase is commonly used as a reporter to assess the transcriptional activity of a genetic construct with luciferase gene under the promoter of interest. Luciferase stable was generated using <b>abm</b>’s <a href=”https://www.abmgood.com/Luciferase (Luc2)-Lentivirus-System-LV010087.html”>pLenti-III-UbC-Luciferase lentivirus</a> on U87MG/ΔEGFR Cells. The expression of luciferase has been confirmed with luciferase assay. All clones are puromycin (0.7 μg/ml)-resistant. Luciferase stable U87MG/ΔEGFR cells can be a very useful cell line for non-invasive visualization in in vitro experiments.</p>
