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mCherry-TPX2 HeLa Stable Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,31 ,African American ,Cervial cancer |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
This HeLa cell line stably expresses targeting protein for Xklp2 (TPX2) fused with mCherry, a fluorescent marker. TPX2 is a critical microtubule-associated protein that plays a pivotal role in mitotic spindle formation by nucleating microtubules and ensuring proper spindle assembly. The stable expression of mCherry-TPX2 in HeLa cells facilitates real-time observation of TPX2 dynamics during mitosis, providing a powerful model for studying spindle formation, chromosome segregation, and the mechanisms underlying mitotic regulation and its implications in cancer.Additional cell line of interest:Cat. T3679 – PRC1-mCherry HeLa Stable Cell Line
