Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
MELA29 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Skin |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,Melanoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow II (TM002) + 5% FBS + 1% NEAA + 1 mM Sodium Pyruvate + 10 mM HEPES + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. Note: Cells are sensitive to freeze/thaw cycles; supplementing media with 20% FBS is recommended during the initial thaw. Once cells recover ,5% FBS is sufficient. Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
This tumor cell line is cultured from patient tumor tissue; cells were passaged 30 times to confirm immortalization. Cell characterization was conducted through STR of the patient tumor and cell line. This cell line is resistant to SSI-4 and expresses wild-type BRAF.
