Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
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Centrifugy
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Real-time PCR cyclery
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Izolátory nukleových kyselin
Pipetovací roboty
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Jednokanálové
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PCR, qPCR, RT
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Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Fetal |
| Growth Properties |
Adherent ,fibroblast-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. DMEM/F-12 (1:1) Medium (TM510) + 10% FBS + 0.1 mM Non-Essential Amino Acids (TM068) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/mitc-inactivated-rat-embryonic-fibroblast-ref-feeder-cells.html |
The inactivated REFs have been tested to be significantly better than inactivated MEF in supporting human iPSC reprogramming and long-term undifferentiated proliferation of human iPS cells and human ground state naive pluripotent stem cells. There is no need to change medium during weekend if human iPS or ES cells are split on Friday and cultured on this REF feeder line in iPSC Culture Medium (TM020).
