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Biochemie
Imunoanalýza
Mitoxantrone-Resistant SF295 Cell Line (MX100)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Brain |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,67 ,Temporal lobe tumor (glioblastoma) |
| Growth Properties |
Adherent ,Neuronal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
https://www.abmgood.com/mitoxantrone-resistant-sf295-cell-line-mx100.html |
The human malignant glioma cell line (SF295) was selected in increasing concentrations of mitoxantrone to yield these cell lines. Cells were maintained in constant culture with mitoxantrone for 3 months before cytogenetic analysis was conducted. MX# indicates the mitoxantrone concentration that the cell line is resistant to in nmol/L. Northern blot analysis was used to confirm ABCG2 expresion in mitoxantrone selected SF295 sublines. The cell lines show increasing ABCG2 expression at the RNA level with increasing mitoxantrone concentration. These cell lines are suitable for studies investigating drug resistance in clinical oncology.
