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MT-IIA Overexpressing H9c2 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Heart |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Embryonic BDIX Rat |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/mt-iia-overexpressing-h9c2-cell-line.html |
Rat heart-derived H9c2 cell line was stably transfected with a vector in which the human MT-IIA gene was placed under the control of the constitutively active beta-actin promoter. The stable MT-IIA over-expressing cardiac cell line is made so that we can understand the molecular mechanisms underlying the role of MT in the heart and evaluate its anti-oxidative property.
