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Mutant Alpha-Synuclein (Ala53Thr) Stable H4 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Brain |
| Organism |
Human (H. sapiens) |
| Donor History | |
| Growth Properties |
Adherent ,fibroblast-like (flat cells with large cytoplasm) |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for cell adhesion to the culture vessels. PriGrow C Medium (TM100) + 10% FBS + 200ug/ml Hygromycin B (G265) + 200ug/ml G418 (G262) + 1 ug/ml tetracycline* + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. Note: cells are better at >80% confluency; cells do not grow well if too sparse. These stable lines were developed to have tet regulatable expression (tet-off); stable expression can be maintained with Hygromycin B (200ug/ml) and G418 (200ug/ml). |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/Mutant-Alpha-Synuclein-Ala53Thr-Stable-H4-Cell-Line-T3458.html |
This cell line stably expresses the disease related mutant (Ala53Thr) of alpha-synuclein (α-synuclein) under tetracycline regulatable expression (tet-off). Therefore, it is useful for studies investigating the role of α-synuclein in Lewy body formation and the development of related neurodegenerative diseases. Complete list of cell lines from this panel:Cat. T3427 – Human Wild-Type Alpha-Synuclein H4 Cell LineCat. T3428 – Full-length Gaussia Luciferase H4 Stable Cell Line (HgLuc) Cat. T3429 – α-Synuclein-Luciferase Bioluminescent Stable H4 Cell Line (H4 S1S2)Cat. T3430 – α-Synuclein-Venus Fluorescent Stable H4 Cell Line (H4 V1S/SV2)Cat. T3441 – Full-length Venus Expressing H4 Stable Cell Line (HgLuc) Cat. T3458 – Mutant Alpha-Synuclein (Ala53Thr) Stable H4 Cell LineCat. T3459 – Mutant Alpha-Synuclein (Ala30Pro) Stable H4 Cell Line
