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Mutant L75P-ApoA-I HepG2 Stable Cell Line (HepG2-L75P-ApoA1)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,15 ,White ,Hepatocellular carcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The Mutant L75P-ApoA-I HepG2 Stable Cell Line (HepG2-L75P-ApoA1) offers a specialized model for examining the pathological impacts of the L75P mutation in Apolipoprotein A-I, which is associated with familial systemic amyloidosis and heightened cellular stress responses. This model uniquely expresses the L75P mutant ApoA-I, which impairs the cell’s stress response by altering the localization and expression of Angiogenin (ANG), a critical protein for cell survival under stress conditions. This cell line is indispensable for research into the mechanisms of amyloidogenic-induced cellular stress and apoptosis, providing insights into therapeutic targets for treating amyloidosis and possibly other related conditions. It also serves as a comparative tool against the wild-type ApoA-I expressing cells to delineate the effects of mutations on protein function.Additional cell lines from this panel: Cat. T3531 – APOA1 Expressing HepG2 Stable Cell Line (HepG2-WT-ApoA1)
