Genetika člověka
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Imunoanalýza
N1G4-Citrine Stable Expressing CHO-K1 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Ovary |
| Organism |
Chinese Hamster (C. griseus) |
| Donor History |
Female ,Adult ,Chinese hamster |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. MEM α ,No Nucleosides Medium (TM512) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 625 µg/ml Geneticin/G418 (G271) and 625 µg/ml Blasticidin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/n1g4-citrine-stable-expressing-cho-k1-cell-line.html |
N1G4-Citrine Stable Expressing CHO-K1 Cell Line was transformed to express the Notch receptor. Notch signalling is a highly conserved pathway, which is used extensively in cell communication to coordinate differentiation and form boundaries between groups of cells. Notch receptors interact with Delta-Serrate-Lag2 ligands at the junctions between cells. The N1G4-Citrine Stable Expressing CHO-K1 Cell Line expresses the Notch1 receptor labelled with a citrine reporter protein. Furthermore, the fusion construct of Notch1 expressed in this cell line was designed for specific binding to the Dll1 ligand to avoid endogenous targets of Notch1 when tracking intracellular activity. This cell line was previously used to study how contact geometry affects signally and would be ideal for further research into cell differentiation and communication.
