N2a Split Superfolder GFP-Tau (N2a-ssGT) Cell Line

Unit	1×10⁶ cells / 1.0 ml
Cell Type	Stable (KO/Expressing) Cells
Tissue Type	Brain
Organism	Mouse (M. musculus)
Donor History	Neuroblastoma
Growth Properties	Adherent , neuronal and amoeboid stem cell-like
Growth Conditions	PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) , High Glucose (TM500) + 10% Heat-inactivated FBS + 2 mM L-Glutamine (G275) + 1 mM Sodium Pyruvate (TM057) + 1% Penicillin/Streptomycin Solution (G255) , 37.0°C , 5% CO₂. 400 µg/ml Geneticin/G418 (G271) and 125 µg/ml Zeocin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.
Immortalization Method
Full Information	https://www.abmgood.com/n2a-split-superfolder-gfp-tau-n2a-ssgt-cell-line.html

Unit

1×10⁶ cells / 1.0 ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Brain

Organism

Mouse (M. musculus)

Donor History

Neuroblastoma

Growth Properties

Adherent

neuronal and amoeboid stem cell-like

Growth Conditions

PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM)

High Glucose (TM500) + 10% Heat-inactivated FBS + 2 mM L-Glutamine (G275) + 1 mM Sodium Pyruvate (TM057) + 1% Penicillin/Streptomycin Solution (G255)

37.0°C

5% CO₂. 400 µg/ml Geneticin/G418 (G271) and 125 µg/ml Zeocin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.

Immortalization Method

Full Information

https://www.abmgood.com/n2a-split-superfolder-gfp-tau-n2a-ssgt-cell-line.html

Applied Biological Materials (abm) je jedním z našich klíčových dodavatelů pro biotechnologický průmysl. Společnost byla založena v roce 2004 a již více než deset let podporuje vědecké objevy v oblasti přírodních věd a vývoje léčiv. Její sídlo se nachází ve Vancouveru v Kanadě. ABM je pro nás neocenitelným partnerem zejména v  oblasti dodávek buněčných kultur, genových a virových vektorů, produktů pro molekulární biologii a PCR, nástrojů pro technologie CRISPR a RNAi, protilátek i základních reagencií pro molekulárně genetické procesy.

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