Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
NDUFS2 Stable Knockout HEK293 Cell Line (HEK293ΔNDUFS2)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties | |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/ndufs2-stable-knockout-hek293-cell-line-hek293dndufs2.html |
This cell line was generated by a CRISPR/Cas9 mediated knockout of NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 (NDUFS2) – one of three protein subunits of interfacial region in human embryonic kidney cell line 293 (HEK293). Protein knockout was verified through Western blot analysis. This cell line, HEK293ΔNDUFS2, grows much slower than the parental cell line and requires adequate media change during growth. Additinally, HEK293ΔNDUFS2 cells show decreased Complex I specific respiration, glycolytic capacity, ATP pool and cell membrane integrity, but shows increased Complex II respiration, ROS generation, apoptosis and necrosis. Treatment with idebenone partially restores growth, ATP pool and oxygen consumption in the knockout cells. This cell line reveals the importance of NDUFS2 for cell growth and metabolism and is suitable for research evaluating the efficacy of mitochondrial therapeutics for bioenergetic capacity.
