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Biochemie
Imunoanalýza
NLK Stable Knockout Human A549 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Lung |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,58 ,Caucasian ,Lung carcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/nlk-stable-knockout-human-a549-cell-line.html |
NLK Stable Knockout Human A549 Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the NLK gene (NM_016231) using the following guide RNA sequence: ACACCATCTTCATCCGGGGT. NLK gene has important implications for Protein Coding and this cell line is useful in the study of transferase activity. This cell line is sequenced verified to be biallelic knockout.
