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Imunoanalýza
OATP1B1 Stable Expressing HEK293 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 250 µg/ml Hygromycin (G265) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/oatp1b3-stable-expressing-hek293-cell-line.html |
A new organic anion transporting polypeptide (OATP) was cloned and expressed; deemed human OATP2. OATP plays an important role in the uptake of a variety of lipophilic anions into the human liver. HEK293 cells were transfected with the genes to express the recombinant OATP2 protein onto the lateral membrane of the cells. OATP1B1 Stable Expressing HEK293 Cell Line presents a good model to study the molecular mechanism behind the uptake of organic anions such as bilirubin conjugates and sulfobromophthalein into the human liver.
