Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
OCRL1 Stable Knockout HK2 Proximal Tubule Cell Line (HK2 OCRL KO -/- )
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History | |
| Growth Properties |
Adhrent ,epithelal (flattened ,rounded) |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/OCRL1-Stable-Knockout-HK2-Proximal-Tubule-Cell-Line-HK2-OCRL-KO-.html |
This HK2 Proximal Tubule Cell Line (HK2 OCRL KO -/-) features a CRISPR/Cas9 mediated stable double knockout of the OCRL gene. Clones were verified by Sanger sequencing and western blot of lysates were used to confirm the absence of the Ocrl1 protein. The control cell line for this catalog is available as Cat. T3325 – Wild Type HK2 Human Proximal Tubule Cell Line. Together, these cell lines are useful for investigating the role of OCRL1 in Lowe syndrome.Additional cell lines of interest:Cat. T3327 – Wild Type 293T Human Kidney Cell LineCat. T3328 – OCRL1 Stable Knockout 293T Human Kidney Cell Line
