Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza
OVCAR-3 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Reproductive system cancer cell lines |
| Organism |
Human |
| Donor History |
High grade ovarian serous adenocarcinoma |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: RPMI 1640 ,w: 2.0 mM stable Glutamine ,w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 20% FBS and 0.01 mg/mL human insulin. Dissociation reagent: Accutase. Seeding density: 2 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
OVCAR-3 cells are a human ovarian cancer cell line established from the malignant ascites of a 60-year-old Caucasian female patient with progressive adenocarcinoma of the ovary, refractory to treatment with cyclophosphamide, adriamycin, and cisplatin. Ovcar 3 cells are used in a wide range of studies including drug resistance, particularly those involving DNA damage response biomarkers, homologous recombination repair, and the overall cell cycle dynamics, cancer cell biology, and gene expression studies.
OVCAR-3 cells are epithelial in morphology and have been characterized by their high in vitro growth potential and their ability to form tumors in immunodeficient mice. These cells express several markers characteristic of ovarian carcinoma and have been utilized extensively to study the biology of ovarian cancer.
OVCAR-3 cells are known to have a complex karyotype, with numerous chromosomal abnormalities that are typical of high-grade serous ovarian carcinomas. They are estrogen receptor-positive, which is relatively rare among ovarian cancer cell lines, and this feature is exploited in studies focusing on hormonal influences on ovarian cancer progression and treatment.
In summary, the OVCAR3 cell line stands as a cornerstone in ovarian cancer research, offering a robust model for studying the complex interplay between hormonal influences, drug resistance, and the genetic underpinnings of high-grade ovarian serous adenocarcinoma.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
