p47ᵖʰᵒˣ Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line

The p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line was derived from primary mouse aortic endothelial cells isolated from the thoracic and abdominal aortas of p47phox knockout mice. The primary cells were immortalized using polyoma middle-sized T-antigen (PmT) method. The p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line is able to retain the properties and phenotype of the parental cells including the expression of common markers of endothelial cells including PECAM1, eNOS, VE-cadherin, and von Willebrand Factor. Another interesting characteristic of this cell line is the decrease in superoxide production due to the lack of p47phox. When tested for the functional responses to physiologically relevant shear stresses, such as laminar shear, the p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line still maintained the ability to form tubes as expected from primary endothelial cells. With these characteristics, the p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line is a useful model in cardiology, particularly in studying vascular biology and pathobiology in vitro. It is also useful in studies on p47phox and its role in superoxide production.