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Imunoanalýza
PD-L1 CRISPR Stable Knockout MC38 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Colon |
| Organism |
Mouse (M. musculus) |
| Donor History |
C57BL6 mouse ,Colon adenocarcinoma cells |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Iscove’s Modified Dulbecco’s Medium (IMDM) (TM505) + 8% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/pd-l1-crispr-stable-knockout-mc38-cell-line.html |
PD-L1 CRISPR Stable Knockout MC38 Cell line is derived from a widely used murine colon carcinoma model, MC38. Guide RNAs (gRNA) carried on a gRNA cloning vector were used to target exon 1 of the mouse CD274 gene encoding Programmed death-ligand 1. Successful knockout of PD-L1 was verified by immunefluorescence after cells were subjected in interferon-gamma. Programmed death-ligand 1 (PD-L1) is known to be frequently expressed on both tumor cells and tumor-infiltrating immune cells and is implicated in the suppression of immune response via the induction of T-cell exhaustion. This line may be valuable for use in drug screen studies or in the investigation of PD-L1 related signaling and function.
