Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
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Centrifugy
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Real-time PCR cyclery
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Izolátory nukleových kyselin
Pipetovací roboty
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Jednokanálové
Multikanálové
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PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
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Intolerance
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Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
PLIN1 Stable Expressing 3T3-L1 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Mouse (M. musculus) |
| Donor History |
Male ,Swiss albino ,Embryo |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% Heat-inactivated FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/plin1-stable-expressing-3t3-l1-cell-line.html |
3T3-L1 cells were stably transfected with the pLXSN-Flag-Plin1 WT to establish the PLIN1 Stable Expressing 3T3-L1 Cell Line. The cells are G418 resistant. Lipid droplet-associated protein (Perilipin 1 or PLIN1) has a role in lipid metabolism and adipocyte lipolysis. This cell line may be used to study processes in lipolysis and lipid metabolism pathway.
