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Imunoanalýza
PML/SP100 Stable Double Knockout HEp-2 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,50 ,African American ,Human papillomavirus-related endocervical adenocarcinoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 5% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 2.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/pml-sp100-stable-double-knockout-hep-2-cell-line.html |
PML-/- cells generated using CRISPR/Cas9 human epithelial type 2 (HEp-2) cells. Considered to originate from a human laryngeal carcinoma allow recognition of over 30 different nuclear and cytoplasmic patterns. These cells were used to study the respective roles of PML and SP100 on the course of HSV replication.
