Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
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Real-time PCR cyclery
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Pipetovací roboty
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Jednokanálové
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PCR, qPCR, RT
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Reverzní transkripce
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Biochemie
Imunoanalýza
PTEN Stable MEF Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
Gelatin Coating Solution (TM063) is required for cell adhesion to the culture vessels. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
PTEN Stable MEF Cell Line were established via the 3T9 protocol. MEF cells were derived from wild-type mouse with stable expression of PTEN. PTEN (Phosphatase and tensin homolog deleted on chromosome 10) is a tumor suppressor gene and when mutated or deleted have been observed in primary human cancers. The PTEN Stable MEF Cell Line may be used alongside with the PTEN Stable Knockout MEF Cell Line (available at abm; Cat. No. T6179) to study PTEN and it’s association with cancer biology and prognosis.
