RAJI Cells
| Unit |
5 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Burkitt Lymphoma cell lines |
| Organism |
Human |
| Donor History |
Burkitt lymphoma |
| Growth Properties |
Suspension |
| Growth Conditions |
Culture medium: RPMI 1640 ,w: 2.0 mM stable Glutamine ,w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% heat-inactivated FBS. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
Raji cells are a line of lymphoblast-like cells established by R.J.V. Pulvertaft in 1963 from Burkitt's lymphoma. These cells are widely used in immunology research due to their high expression of human CD19, which acts as a co-receptor and decreases the threshold for antigen B-cell receptor (BCR) stimulation. Raji cells are non-adherent and grow in suspension as free-floating individuals or doublets.
The doubling time of these cells is 23.2 hours, and they are relatively small in diameter with a diameter range of 5-8 μm. Some characteristics of Raji cells include a lack of differentiation, as they form large aggregations of hundreds of individual cells. These cells are diploid and have a stable karyotype within the male diploid stemline of 46.
Additionally, Raji cells are partially resistant to poliovirus and vesicular stomatitis viruses. Human CD19 is highly expressed by Raji cells and has been identified as a clinical target for anti-hCD19-CD3 bis-specific antibodies in non-Hodgkin's B cell lymphoma. BCMA expression has also been identified in the Raji Burkitt lymphoma cell line and primary lymphoma, making it an important area of research for immunologists.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

