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Imunoanalýza
RanBPM ShRNA Stable Knockout HeLa Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,31 ,Cervical carcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% Heat-inactivated FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/ranbpm-shrna-stable-knockout-hela-cell-line.html |
This cell line was generated using small interfering RNA (siRNA) -mediated downregulation of RanBPM. RanBPM has been shown to function as an activator of apoptotic pathways induced by DNA damage. It has been suggested that RanBPM could be an important regulator of pathways that prevent tumorigenesis by promoting the elimination of cells with genomic alterations.
