RFP Stably Expressing THP-1 Cell Line

Unit	1×10⁶ cells / 1.0 ml
Cell Type	Stable (KO/Expressing) Cells
Tissue Type	Peripheral Blood
Organism	Human (H. sapiens)
Donor History	Male , 1 year , Acute monocytic leukemia
Growth Properties	Suspension , round
Growth Conditions	PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. PriGrow XV Medium (TM027) + 1% Penicillin/Streptomycin Solution (G255) , 37.0°C , 5% CO₂.1.2 µg/ml Puromycin (G264) for selection. Note 1: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. Note 2: cells take additional time to recover from freeze-thaw conditions. Allow cells to recover during the first week post-thaw by allowing cells to grow undisturbed. Once cells recover , they will expand quickly.
Immortalization Method
Full Information	https://www.abmgood.com/rfp-stably-expressing-thp-1-cell-line.html

Unit

1×10⁶ cells / 1.0 ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Peripheral Blood

Organism

Human (H. sapiens)

Donor History

Male

1 year

Acute monocytic leukemia

Growth Properties

Suspension

round

Growth Conditions

PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. PriGrow XV Medium (TM027) + 1% Penicillin/Streptomycin Solution (G255)

37.0°C

5% CO₂.1.2 µg/ml Puromycin (G264) for selection. Note 1: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. Note 2: cells take additional time to recover from freeze-thaw conditions. Allow cells to recover during the first week post-thaw by allowing cells to grow undisturbed. Once cells recover

they will expand quickly.

Immortalization Method

Full Information

https://www.abmgood.com/rfp-stably-expressing-thp-1-cell-line.html

Applied Biological Materials (abm) je jedním z našich klíčových dodavatelů pro biotechnologický průmysl. Společnost byla založena v roce 2004 a již více než deset let podporuje vědecké objevy v oblasti přírodních věd a vývoje léčiv. Její sídlo se nachází ve Vancouveru v Kanadě. ABM je pro nás neocenitelným partnerem zejména v  oblasti dodávek buněčných kultur, genových a virových vektorů, produktů pro molekulární biologii a PCR, nástrojů pro technologie CRISPR a RNAi, protilátek i základních reagencií pro molekulárně genetické procesy.

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