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SEC Component FLAG-AF1 Stable Expressing HeLa S3 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,31 ,Cervical carcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 100 µg/ml Geneticin/G418 (G271) and 100 µg/ml Hygromycin (G265) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/sec-component-flag-af1-stable-expressing-hela-s3-cell-line.html |
HeLa S3 cells are derived from the parental HeLa cell line. To establish this stable cell line; mCAT1 was transfected into the HeLa S3 cells using FuGene HD. The SEC component FLAG-AF1 was then transduced into the HeLa-S3-mCAT1 cells via Ecotropic retrovirus system. This cell line is G418 and hygromycin resistant and can be used in DNA transcription studies.
