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SIAT1 Stable Expressing MDCK (MDCK-SIAT1) Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Dog (Canine) |
| Donor History |
Female ,Adult |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 mg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/siat1-stable-expressing-mdck-mdck-siat1-cell-line.html |
The SIAT1 Stable Expressing MDCK (MDCK-SIAT1) Cell Line was generated by stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). When compared to the parental MDCK cells, the MDCK-SIAT1 is able to express increased levels of α-2,6-linked sialic acid receptors for isolation of circulating influenza virus. The SIAT1 Stable Expressing MDCK (MDCK-SIAT1) Cell Line is useful in isolation and propagation of human influenza viruses for observation, diagnostic, and research purposes.If use of this culture results in a scientific publication, it should be cited in the publication as: MDCK-SIAT1 (ECACC 05071502).
