Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Stable GS-3A4-HepG2 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,15 ,White ,Hepatocellular carcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Mix 1:1 PriGrow II (TM002) and PriGrow IV (TM004) + 10% dialyzed FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂ |
| Immortalization Method | |
| Full Information |
The parental cell line of GS-3A4-HepG2 (HepG2) was derived from a human hepatoma and was transformed with a vector containing the Glutamine Synthetase gene allowing for ammonia metabolism and the 3A4 gene for drug metabolism. This transformation allows for activity in the immortalized cell line that is similar to primary hepatocytes but with a greater ability to metabolise drugs (3A4 activity). This cell line is highly functional in long term culture and therefore ideal for continuous research into liver function and metabolism. Previous studies have explored the cell lines use into bioartificial liver support systems. These systems are new technologies being developed to treat cases of liver damage where function needs to be supported during liver regeneration. The GS-3A4-HepG2 cell line may be particularly useful to metabolism drugs during long term experiments.
