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Imunoanalýza
Stable Rab37 Knockdown H460 Lung Cancer Cell Line (shRab37#1)
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Lung |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,Carcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 2.5 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-rab37-knockdown-h460-lung-cancer-cell-line-shrab37-1.html |
Rab37 knockdown was acheived via transduction of the H460 cells with lentivirus to establish Stable Rab37 Knockdown H460 Lung Cancer Cell Line (shRab37#1). Another clone, Stable Rab37 Knockdown H460 Lung Cancer Cell Line (shRab37#2) Cat. No. T6326 is also available for use to study Rab small GTPase, Rab37.
