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Stable TIM3 Expressing CHO-K1 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Ovary |
| Organism |
Chinese Hamster (C. griseus) |
| Donor History |
Female ,Adult ,Chinese hamster |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. DMEM/F-12 (1:1) Medium (TM510) + 10% FBS + 4 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 30 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-tim3-expressing-cho-k1-cell-line.html |
CHO-K1 Cells were transfected with HAVCR2 Lentiviral Vector (pLenti-GIII-CMV-GFP-2A-Puro) (LV710795) to generate the Stable TIM3 Expressing CHO-K1 Cell Line. The cells were selected against puromycin-resistance and display adherent growth property with epithelial morphology. The Stable TIM3 Expressing CHO-K1 Cell Line is also positive for GFP-expression. The cells can be used in studies focusing on HAVCR2 gene and the TIM3 pathway with its interaction with the PD1 pathway. Other applications for the Stable TIM3 Expressing CHO-K1 Cell Line include positive control for western blot or binding assays.
