Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
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Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
SUNE2 Cells
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Airway |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,38 ,Asian ,nasopharyngeal carcinoma (Stage III undifferentiated squamous cell carcinoma) |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
The SUNE2 cell line is derived from a nasopharyngeal carcinoma (NPC) from the biopsy performed on a 38-year-old female Cantonese patient. This cell line has extremely low concentrations of Epstein-Barr virus (EBV) DNA in long-term culture and expressed low levels of latent membrane protein 1 (LMP1), latent membrane protein 2A (LMP2A), BamH1-A right frame 1 (BARF1), EBV-encoded RNA-1 (EBER1), and EBV-encoded RNA-2 (EBER2) in early passages. The SUNE2 cells also show a significantly stronger transforming ability than the control 5-8F cells in colony where it only requires 1 week for a single SUNE2 cell to form a colony of more than 200 cells. The SUNE2 cells are also able to form tumors in nude mice. As the proliferation of SUNE2 cells is significantly faster than other NPC cell lines, SUNE2 cell line is considered to be a new in vitro model which is highly valuable in research on the mechanisms underlying the occurrence and development of NPC.
