SW620-GFP Cells

SW620-GFP cells are a fluorescently labeled variant of the human colorectal adenocarcinoma cell line SW620, which was originally derived from a metastatic lesion in a lymph node of an adult patient. These cells have been engineered to stably express green fluorescent protein (GFP), enabling direct visualization of cellular morphology, proliferation, and migratory behavior in real time using fluorescence-based imaging techniques. The parental SW620 line is characterized by its metastatic origin and exhibits features associated with advanced colorectal cancer, including enhanced motility, invasive capacity, and altered cell adhesion properties.

The expression of GFP in SW620-GFP cells facilitates applications such as live-cell tracking, invasion assays, and in vivo imaging in xenograft models, where tumor growth and dissemination can be monitored non-invasively. These cells retain key molecular characteristics of the parental line, including mutations commonly associated with colorectal cancer progression and dysregulation of signaling pathways such as Wnt/β-catenin and MAPK. As a result, SW620-GFP cells are a valuable tool for studying mechanisms of metastasis, tumor microenvironment interactions, and the evaluation of anti-cancer therapeutics targeting advanced-stage colorectal carcinoma.

Genetic modification: Stably modified by replication-incompetent lentiviral transduction to express the ZsGreen1 green fluorescent protein reporter; maintained as a polyclonal population under puromycin selection (1–5 µg/mL). S1/BSL-1 containment.