TK6 Cells

Unit

5 x 10^6 cells/ml

Cell Type

Immortalized Cells

Tissue Type

Leukemia cell lines

Organism

Human

Donor History
Growth Properties

Suspension

Growth Conditions

Culture medium: RPMI 1640

,

w: 2.0 mM stable Glutamine

,

w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with heat inactivated 10% FBS

,

2

,

5% horse serum. Seeding density: 1 x 10⁵ cells/mL. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/TK6-Cells/300357

SKU: 300357 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

TK6 is a lymphoblast cell line derived from the spleen of a 5-year-old male diagnosed with hereditary spherocytosis. This cell line is particularly notable for being heterozygous at the thymidine kinase (TK) locus, which underpins its utility in genetic research. The heterozygosity at the TK locus allows the TK6 cells to serve as a sensitive model for detecting forward mutations, providing a robust platform for mutagenicity testing and genetic toxicology studies.

The cells are employed extensively in assays designed to quantitatively detect forward mutations at three loci, including resistance to trifluorothymidine at the tk locus. This capability makes TK6 an invaluable tool in the pharmaceutical and chemical industries for evaluating the mutagenic potential of new compounds. The cell line’s unique genetic background and its relevance to disease make it a critical resource for studies focused on understanding mutation processes and evaluating the cytogenetic effects of chemical exposures in a controlled environment.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.