TLR4 & CD14 Stable Expressing CHO Cell Line (3E10 TLR4)

Unit	1×10⁶ cells / 1 ml
Cell Type	Stable (KO/Expressing) Cells
Tissue Type	Ovary
Organism	Chinese Hamster (C. griseus)
Donor History	Female , Adult , Chinese hamster
Growth Properties	Adherent , epithelial
Growth Conditions	PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Ham's F-12 Medium + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) , 37.0°C , 5% CO₂. 400 µg/ml Hygromycin (G265) , 50 µg/ml Puromycin (G264) , and 10 μg/ml Ciprofloxacin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.
Immortalization Method
Full Information	https://www.abmgood.com/tlr4-cd14-stable-expressing-cho-cell-line-3e10-tlr4.html

Unit

1×10⁶ cells / 1 ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Ovary

Organism

Chinese Hamster (C. griseus)

Donor History

Female

Adult

Chinese hamster

Growth Properties

Adherent

epithelial

Growth Conditions

PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Ham's F-12 Medium + 10% FBS + 1% Penicillin/Streptomycin Solution (G255)

37.0°C

5% CO₂. 400 µg/ml Hygromycin (G265)

50 µg/ml Puromycin (G264)

and 10 μg/ml Ciprofloxacin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.

Immortalization Method

Full Information

https://www.abmgood.com/tlr4-cd14-stable-expressing-cho-cell-line-3e10-tlr4.html

Applied Biological Materials (abm) je jedním z našich klíčových dodavatelů pro biotechnologický průmysl. Společnost byla založena v roce 2004 a již více než deset let podporuje vědecké objevy v oblasti přírodních věd a vývoje léčiv. Její sídlo se nachází ve Vancouveru v Kanadě. ABM je pro nás neocenitelným partnerem zejména v  oblasti dodávek buněčných kultur, genových a virových vektorů, produktů pro molekulární biologii a PCR, nástrojů pro technologie CRISPR a RNAi, protilátek i základních reagencií pro molekulárně genetické procesy.

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