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U251MG Stably Expressing Tet-on Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Brain |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,75 ,Caucasian ,Astrocytoma |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. DMEM/F-12 (1:1) Medium (TM510) + 10% Tet-tested FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 200 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/u251mg-stably-expressing-tet-on-cell-line.html |
U251MG cells were stably transfected with the rtTA expression vector to generate the U251MG Stably Expressing Tet-on Cell Line. It can be used in vitro to stably transfect gene of interest cloned in tetracycline-responsive expression vector (i.e. pTRE2 with Hygromycin selection) and pick clones. With the addition of 1 µg/ml doxycycline, end users can test individually for induced gene expression under the tet-regulated promoter.
