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Biochemie
Imunoanalýza
UBXN1 Stable Knockout HeLa Cell Line – Clone 71
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,31 ,Cervical carcinoma |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1 mM Sodium Pyruvate (TM057) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/UBXN1-Stable-Knockout-HeLa-Cell-Line-Clone-71-t3301.html |
These HeLa cells feature a stable knockout of the VCP cofactor UBA and UBX domain-containing UBXN1/SAKS1. UBXN1 colocalizes with ubiquitin and is involved in ubiquitin-proteasome functions. The cofactor plays an imporant role in mitochondrial fragmentation, ATP production, and ER-mitochondrial apposition. Knockout was confirmed via Western blot. The cell line is a suitable model for research regarding mitophagy. This cell line is available as two clones (Cat. T3301 and T3310).
