Scrambled-peptide Knockdown Control Cell Line Expressing Dendra2
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Mammary |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Female ,Fischer 344 rat |
| Growth Properties |
Adherent ,round to flat |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow VIII (TM018) + 5% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. Selection with 1 mg/ml G418 (G271) and 10 µg/ml Puromycin (G264) |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/scrambled-peptide-knockdown-control-cell-line-expressing-dendra2.html |
Lipofectamine2000 transfection of Dendra2 plasmid (pDendra2-N ) with photoconvertible fluorescent marker in MTLn3 cells followed by selection for stable Dendra2 expression using 1 mg/ml geneticin. Lipofectamine2000 transfection of the pSUPERretro SCR plasmid into MTLn3 Dendra2 cells followed by selection for stable expression of SCR shRNA using 10 µg/ml puromycin.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
